IFCC approved HPLC reference measurement procedure for the alcohol consumption biomarker carbohydrate-deficient transferrin (CDT): Its validation and use.
Identifieur interne : 000A53 ( Main/Exploration ); précédent : 000A52; suivant : 000A54IFCC approved HPLC reference measurement procedure for the alcohol consumption biomarker carbohydrate-deficient transferrin (CDT): Its validation and use.
Auteurs : François Schellenberg [France] ; Jos Wielders [Pays-Bas] ; Raymond Anton [États-Unis] ; Vincenza Bianchi [Italie] ; Jean Deenmamode [Royaume-Uni] ; Cas Weykamp [Pays-Bas] ; John Whitfield [Australie] ; Jan-Olof Jeppsson [Suède] ; Anders Helander [Suède]Source :
- Clinica chimica acta; international journal of clinical chemistry [ 1873-3492 ] ; 2017.
Descripteurs français
- KwdFr :
- Calibrage, Chromatographie en phase liquide à haute performance (), Chromatographie en phase liquide à haute performance (instrumentation), Chromatographie en phase liquide à haute performance (normes), Consommation d'alcool (sang), Contrôle de qualité, Humains, Limite de détection, Marqueurs biologiques (sang), Normes de référence, Transferrine (analogues et dérivés), Transferrine (analyse), Transferrine (génétique), Valeurs de référence, Électrophorèse capillaire ().
- MESH :
- analogues et dérivés : Transferrine.
- analyse : Transferrine.
- génétique : Transferrine.
- normes : Chromatographie en phase liquide à haute performance.
- sang : Chromatographie en phase liquide à haute performance, Consommation d'alcool, Marqueurs biologiques.
- Calibrage, Chromatographie en phase liquide à haute performance, Contrôle de qualité, Humains, Limite de détection, Normes de référence, Valeurs de référence, Électrophorèse capillaire.
English descriptors
- KwdEn :
- Alcohol Drinking (blood), Biomarkers (blood), Calibration, Chromatography, High Pressure Liquid (instrumentation), Chromatography, High Pressure Liquid (methods), Chromatography, High Pressure Liquid (standards), Electrophoresis, Capillary (methods), Humans, Limit of Detection, Quality Control, Reference Standards, Reference Values, Transferrin (analogs & derivatives), Transferrin (analysis), Transferrin (genetics).
- MESH :
- chemical , analogs & derivatives : Transferrin.
- chemical , analysis : Transferrin.
- chemical , blood : Biomarkers.
- blood : Alcohol Drinking.
- chemical , genetics : Transferrin.
- instrumentation : Chromatography, High Pressure Liquid.
- methods : Chromatography, High Pressure Liquid, Electrophoresis, Capillary.
- standards : Chromatography, High Pressure Liquid.
- Calibration, Humans, Limit of Detection, Quality Control, Reference Standards, Reference Values.
Abstract
Carbohydrate-deficient transferrin (CDT) is used as a biomarker of sustained high alcohol consumption. The currently available measurement procedures for CDT are based on various analytical techniques (HPLC, capillary electrophoresis, nephelometry), some differing in the definition of the analyte and using different reference intervals and cut-off values. The Working Group on Standardization of CDT (WG-CDT), initiated by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), has validated an HPLC candidate reference measurement procedure (cRMP) for CDT (% disialotransferrin to total transferrin based on peak areas), demonstrating that it is suitable as a reference measurement procedure (RMP) for CDT. Presented is a detailed description of the cRMP and its calibration. Practical aspects on how to treat genetic variant and so-called di-tri bridge samples are described. Results of method performance characteristics, as demanded by ISO 15189 and ISO 15193, are given, as well as the reference interval and measurement uncertainty and how to deal with that in routine use. The correlation of the cRMP with commercial CDT procedures and the performance of the cRMP in a network of laboratories are also presented. The performance of the CDT cRMP in combination with previously developed commutable calibrators allows for standardization of the currently available commercial measurement procedures for CDT. The cRMP has recently been approved by the IFCC and will be from now on be known as the IFCC-RMP for CDT, while CDT results standardized according to this RMP should be indicated as CDTIFCC.
DOI: 10.1016/j.cca.2016.12.022
PubMed: 28025028
Affiliations:
- Australie, France, Italie, Pays-Bas, Royaume-Uni, Suède, États-Unis
- Angleterre, Caroline du Sud, Centre-Val de Loire, Grand Londres, Région Centre, Svealand
- Londres, Stockholm, Tours
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001097
- to stream PubMed, to step Curation: 001094
- to stream PubMed, to step Checkpoint: 001094
- to stream Ncbi, to step Merge: 004090
- to stream Ncbi, to step Curation: 004090
- to stream Ncbi, to step Checkpoint: 004090
- to stream Main, to step Merge: 000A48
- to stream Main, to step Curation: 000A53
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">IFCC approved HPLC reference measurement procedure for the alcohol consumption biomarker carbohydrate-deficient transferrin (CDT): Its validation and use.</title><author><name sortKey="Schellenberg, Francois" sort="Schellenberg, Francois" uniqKey="Schellenberg F" first="François" last="Schellenberg">François Schellenberg</name><affiliation wicri:level="3"><nlm:affiliation>Hôpital Trousseau, CHRU, Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Hôpital Trousseau, CHRU, Tours</wicri:regionArea><placeName><region type="region">Centre-Val de Loire</region><region type="old region">Région Centre</region><settlement type="city">Tours</settlement></placeName></affiliation></author><author><name sortKey="Wielders, Jos" sort="Wielders, Jos" uniqKey="Wielders J" first="Jos" last="Wielders">Jos Wielders</name><affiliation wicri:level="1"><nlm:affiliation>Meander Medisch Centrum, Amersfoort, The Netherlands. Electronic address: joswielders@gmail.com.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Meander Medisch Centrum, Amersfoort</wicri:regionArea><wicri:noRegion>Amersfoort</wicri:noRegion></affiliation></author><author><name sortKey="Anton, Raymond" sort="Anton, Raymond" uniqKey="Anton R" first="Raymond" last="Anton">Raymond Anton</name><affiliation wicri:level="2"><nlm:affiliation>Medical University of South Carolina, Charleston, SC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Medical University of South Carolina, Charleston, SC</wicri:regionArea><placeName><region type="state">Caroline du Sud</region></placeName></affiliation></author><author><name sortKey="Bianchi, Vincenza" sort="Bianchi, Vincenza" uniqKey="Bianchi V" first="Vincenza" last="Bianchi">Vincenza Bianchi</name><affiliation wicri:level="1"><nlm:affiliation>SS. Antonio e Biagio Hospital, Alessandria, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>SS. Antonio e Biagio Hospital, Alessandria</wicri:regionArea><wicri:noRegion>Alessandria</wicri:noRegion></affiliation></author><author><name sortKey="Deenmamode, Jean" sort="Deenmamode, Jean" uniqKey="Deenmamode J" first="Jean" last="Deenmamode">Jean Deenmamode</name><affiliation wicri:level="3"><nlm:affiliation>Homerton University Hospital, London, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Homerton University Hospital, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Weykamp, Cas" sort="Weykamp, Cas" uniqKey="Weykamp C" first="Cas" last="Weykamp">Cas Weykamp</name><affiliation wicri:level="1"><nlm:affiliation>Queen Beatrix Hospital, Winterswijk, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Queen Beatrix Hospital, Winterswijk</wicri:regionArea><wicri:noRegion>Winterswijk</wicri:noRegion></affiliation></author><author><name sortKey="Whitfield, John" sort="Whitfield, John" uniqKey="Whitfield J" first="John" last="Whitfield">John Whitfield</name><affiliation wicri:level="1"><nlm:affiliation>Queensland Institute of Medical Research, Brisbane, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Queensland Institute of Medical Research, Brisbane</wicri:regionArea><wicri:noRegion>Brisbane</wicri:noRegion></affiliation></author><author><name sortKey="Jeppsson, Jan Olof" sort="Jeppsson, Jan Olof" uniqKey="Jeppsson J" first="Jan-Olof" last="Jeppsson">Jan-Olof Jeppsson</name><affiliation wicri:level="1"><nlm:affiliation>Skåne University Hospital, Malmö, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Skåne University Hospital, Malmö</wicri:regionArea><wicri:noRegion>Malmö</wicri:noRegion></affiliation></author><author><name sortKey="Helander, Anders" sort="Helander, Anders" uniqKey="Helander A" first="Anders" last="Helander">Anders Helander</name><affiliation wicri:level="3"><nlm:affiliation>Karolinska Institutet, Karolinska University Laboratory, Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Karolinska Institutet, Karolinska University Laboratory, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28025028</idno><idno type="pmid">28025028</idno><idno type="doi">10.1016/j.cca.2016.12.022</idno><idno type="wicri:Area/PubMed/Corpus">001097</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001097</idno><idno type="wicri:Area/PubMed/Curation">001094</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001094</idno><idno type="wicri:Area/PubMed/Checkpoint">001094</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001094</idno><idno type="wicri:Area/Ncbi/Merge">004090</idno><idno type="wicri:Area/Ncbi/Curation">004090</idno><idno type="wicri:Area/Ncbi/Checkpoint">004090</idno><idno type="wicri:Area/Main/Merge">000A48</idno><idno type="wicri:Area/Main/Curation">000A53</idno><idno type="wicri:Area/Main/Exploration">000A53</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">IFCC approved HPLC reference measurement procedure for the alcohol consumption biomarker carbohydrate-deficient transferrin (CDT): Its validation and use.</title><author><name sortKey="Schellenberg, Francois" sort="Schellenberg, Francois" uniqKey="Schellenberg F" first="François" last="Schellenberg">François Schellenberg</name><affiliation wicri:level="3"><nlm:affiliation>Hôpital Trousseau, CHRU, Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Hôpital Trousseau, CHRU, Tours</wicri:regionArea><placeName><region type="region">Centre-Val de Loire</region><region type="old region">Région Centre</region><settlement type="city">Tours</settlement></placeName></affiliation></author><author><name sortKey="Wielders, Jos" sort="Wielders, Jos" uniqKey="Wielders J" first="Jos" last="Wielders">Jos Wielders</name><affiliation wicri:level="1"><nlm:affiliation>Meander Medisch Centrum, Amersfoort, The Netherlands. Electronic address: joswielders@gmail.com.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Meander Medisch Centrum, Amersfoort</wicri:regionArea><wicri:noRegion>Amersfoort</wicri:noRegion></affiliation></author><author><name sortKey="Anton, Raymond" sort="Anton, Raymond" uniqKey="Anton R" first="Raymond" last="Anton">Raymond Anton</name><affiliation wicri:level="2"><nlm:affiliation>Medical University of South Carolina, Charleston, SC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Medical University of South Carolina, Charleston, SC</wicri:regionArea><placeName><region type="state">Caroline du Sud</region></placeName></affiliation></author><author><name sortKey="Bianchi, Vincenza" sort="Bianchi, Vincenza" uniqKey="Bianchi V" first="Vincenza" last="Bianchi">Vincenza Bianchi</name><affiliation wicri:level="1"><nlm:affiliation>SS. Antonio e Biagio Hospital, Alessandria, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>SS. Antonio e Biagio Hospital, Alessandria</wicri:regionArea><wicri:noRegion>Alessandria</wicri:noRegion></affiliation></author><author><name sortKey="Deenmamode, Jean" sort="Deenmamode, Jean" uniqKey="Deenmamode J" first="Jean" last="Deenmamode">Jean Deenmamode</name><affiliation wicri:level="3"><nlm:affiliation>Homerton University Hospital, London, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Homerton University Hospital, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Weykamp, Cas" sort="Weykamp, Cas" uniqKey="Weykamp C" first="Cas" last="Weykamp">Cas Weykamp</name><affiliation wicri:level="1"><nlm:affiliation>Queen Beatrix Hospital, Winterswijk, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Queen Beatrix Hospital, Winterswijk</wicri:regionArea><wicri:noRegion>Winterswijk</wicri:noRegion></affiliation></author><author><name sortKey="Whitfield, John" sort="Whitfield, John" uniqKey="Whitfield J" first="John" last="Whitfield">John Whitfield</name><affiliation wicri:level="1"><nlm:affiliation>Queensland Institute of Medical Research, Brisbane, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Queensland Institute of Medical Research, Brisbane</wicri:regionArea><wicri:noRegion>Brisbane</wicri:noRegion></affiliation></author><author><name sortKey="Jeppsson, Jan Olof" sort="Jeppsson, Jan Olof" uniqKey="Jeppsson J" first="Jan-Olof" last="Jeppsson">Jan-Olof Jeppsson</name><affiliation wicri:level="1"><nlm:affiliation>Skåne University Hospital, Malmö, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Skåne University Hospital, Malmö</wicri:regionArea><wicri:noRegion>Malmö</wicri:noRegion></affiliation></author><author><name sortKey="Helander, Anders" sort="Helander, Anders" uniqKey="Helander A" first="Anders" last="Helander">Anders Helander</name><affiliation wicri:level="3"><nlm:affiliation>Karolinska Institutet, Karolinska University Laboratory, Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Karolinska Institutet, Karolinska University Laboratory, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author></analytic><series><title level="j">Clinica chimica acta; international journal of clinical chemistry</title><idno type="eISSN">1873-3492</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alcohol Drinking (blood)</term><term>Biomarkers (blood)</term><term>Calibration</term><term>Chromatography, High Pressure Liquid (instrumentation)</term><term>Chromatography, High Pressure Liquid (methods)</term><term>Chromatography, High Pressure Liquid (standards)</term><term>Electrophoresis, Capillary (methods)</term><term>Humans</term><term>Limit of Detection</term><term>Quality Control</term><term>Reference Standards</term><term>Reference Values</term><term>Transferrin (analogs & derivatives)</term><term>Transferrin (analysis)</term><term>Transferrin (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Calibrage</term><term>Chromatographie en phase liquide à haute performance ()</term><term>Chromatographie en phase liquide à haute performance (instrumentation)</term><term>Chromatographie en phase liquide à haute performance (normes)</term><term>Consommation d'alcool (sang)</term><term>Contrôle de qualité</term><term>Humains</term><term>Limite de détection</term><term>Marqueurs biologiques (sang)</term><term>Normes de référence</term><term>Transferrine (analogues et dérivés)</term><term>Transferrine (analyse)</term><term>Transferrine (génétique)</term><term>Valeurs de référence</term><term>Électrophorèse capillaire ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Transferrin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Transferrin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Biomarkers</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Transferrine</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Transferrine</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Alcohol Drinking</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Transferrin</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Transferrine</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Chromatography, High Pressure Liquid</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Electrophoresis, Capillary</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term><term>Consommation d'alcool</term><term>Marqueurs biologiques</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Chromatography, High Pressure Liquid</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Calibration</term><term>Humans</term><term>Limit of Detection</term><term>Quality Control</term><term>Reference Standards</term><term>Reference Values</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Calibrage</term><term>Chromatographie en phase liquide à haute performance</term><term>Contrôle de qualité</term><term>Humains</term><term>Limite de détection</term><term>Normes de référence</term><term>Valeurs de référence</term><term>Électrophorèse capillaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Carbohydrate-deficient transferrin (CDT) is used as a biomarker of sustained high alcohol consumption. The currently available measurement procedures for CDT are based on various analytical techniques (HPLC, capillary electrophoresis, nephelometry), some differing in the definition of the analyte and using different reference intervals and cut-off values. The Working Group on Standardization of CDT (WG-CDT), initiated by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), has validated an HPLC candidate reference measurement procedure (cRMP) for CDT (% disialotransferrin to total transferrin based on peak areas), demonstrating that it is suitable as a reference measurement procedure (RMP) for CDT. Presented is a detailed description of the cRMP and its calibration. Practical aspects on how to treat genetic variant and so-called di-tri bridge samples are described. Results of method performance characteristics, as demanded by ISO 15189 and ISO 15193, are given, as well as the reference interval and measurement uncertainty and how to deal with that in routine use. The correlation of the cRMP with commercial CDT procedures and the performance of the cRMP in a network of laboratories are also presented. The performance of the CDT cRMP in combination with previously developed commutable calibrators allows for standardization of the currently available commercial measurement procedures for CDT. The cRMP has recently been approved by the IFCC and will be from now on be known as the IFCC-RMP for CDT, while CDT results standardized according to this RMP should be indicated as CDTIFCC.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>Italie</li><li>Pays-Bas</li><li>Royaume-Uni</li><li>Suède</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Caroline du Sud</li><li>Centre-Val de Loire</li><li>Grand Londres</li><li>Région Centre</li><li>Svealand</li></region><settlement><li>Londres</li><li>Stockholm</li><li>Tours</li></settlement></list><tree><country name="France"><region name="Centre-Val de Loire"><name sortKey="Schellenberg, Francois" sort="Schellenberg, Francois" uniqKey="Schellenberg F" first="François" last="Schellenberg">François Schellenberg</name></region></country><country name="Pays-Bas"><noRegion><name sortKey="Wielders, Jos" sort="Wielders, Jos" uniqKey="Wielders J" first="Jos" last="Wielders">Jos Wielders</name></noRegion><name sortKey="Weykamp, Cas" sort="Weykamp, Cas" uniqKey="Weykamp C" first="Cas" last="Weykamp">Cas Weykamp</name></country><country name="États-Unis"><region name="Caroline du Sud"><name sortKey="Anton, Raymond" sort="Anton, Raymond" uniqKey="Anton R" first="Raymond" last="Anton">Raymond Anton</name></region></country><country name="Italie"><noRegion><name sortKey="Bianchi, Vincenza" sort="Bianchi, Vincenza" uniqKey="Bianchi V" first="Vincenza" last="Bianchi">Vincenza Bianchi</name></noRegion></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Deenmamode, Jean" sort="Deenmamode, Jean" uniqKey="Deenmamode J" first="Jean" last="Deenmamode">Jean Deenmamode</name></region></country><country name="Australie"><noRegion><name sortKey="Whitfield, John" sort="Whitfield, John" uniqKey="Whitfield J" first="John" last="Whitfield">John Whitfield</name></noRegion></country><country name="Suède"><noRegion><name sortKey="Jeppsson, Jan Olof" sort="Jeppsson, Jan Olof" uniqKey="Jeppsson J" first="Jan-Olof" last="Jeppsson">Jan-Olof Jeppsson</name></noRegion><name sortKey="Helander, Anders" sort="Helander, Anders" uniqKey="Helander A" first="Anders" last="Helander">Anders Helander</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A53 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000A53 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:28025028
|texte= IFCC approved HPLC reference measurement procedure for the alcohol consumption biomarker carbohydrate-deficient transferrin (CDT): Its validation and use.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:28025028" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |